Potential DNA barcodes for Melilotus species based on five single loci and their combinations

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Potential DNA barcodes for Melilotus species based on five single loci and their combinations

Melilotus, an annual or biennial herb, belongs to the tribe Trifolieae (Leguminosae) and consists of 19 species. As an important green manure crop, diverse Melilotus species have different values as feed and medicine. To identify different Melilotus species, we examined the efficiency of five candidate regions as barcodes, including the internal transcribed spacer (ITS) and two chloroplast loci...

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DNA barcodes for biosecurity: invasive species identification.

Biosecurity encompasses protecting against any risk through 'biological harm', not least being the economic impact from the spread of pest insects. Molecular diagnostic tools provide valuable support for the rapid and accurate identification of morphologically indistinct alien species. However, these tools currently lack standardization. They are not conducive to adaptation by multiple sectors ...

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SPECIES OF MELILOTUS IN IRAN (KEY TO THE SPECIES, DESCRIPTIONS AND THEIR DISTRIBUTIONS)

The genus Melilotus contains altogether some 20 species (WIERSEMA et al. 1990) in the world which mostly distributed in Europe, Central and SW. Asia and N. Africa (HUCHINSON 1964). BOISSIER (1872) listed 13 species of Melilotus in which 6 of them are found in the Iranian flora. PARSA (1948) following Boissier’s work referring some new collections made by other workers, listed the same species n...

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Evaluation of seven DNA barcodes for differentiating closely related medicinal Gentiana species and their adulterants

BACKGROUND Species identification of living organisms by standard DNA sequences has been well-accepted. Consortium for the Barcode of Life (CBOL) recommends chloroplast regions rbcL and matK as the DNA barcodes for the land plants. This study aims to evaluate the feasibility and limitations of rbcL, matK, and 5 other commonly used regions as the DNA barcodes for the medicinal Gentiana and their...

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DNA barcodes distinguish species of tropical Lepidoptera.

Although central to much biological research, the identification of species is often difficult. The use of DNA barcodes, short DNA sequences from a standardized region of the genome, has recently been proposed as a tool to facilitate species identification and discovery. However, the effectiveness of DNA barcoding for identifying specimens in species-rich tropical biotas is unknown. Here we sho...

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ژورنال

عنوان ژورنال: PLOS ONE

سال: 2017

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0182693